Pelgipeptin L, a new type of pelgipeptin family isolated from <em>Paenibacillus elgii</em> — ASN Events
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Pelgipeptin L, a new type of pelgipeptin family isolated from Paenibacillus elgii (#103)

Jueun Kim 1 , Chul Won Lee 1
  1. chonnam national university in korea, Chonnam National University/ Chemistry, South Korea

Pelgipeptins are cyclic lipopeptides composed of nine amino acids with a short hydrophobic fatty acid chain. To date, pelgipeptin A, B, C, and D have been identified. Their structures are distinguished by amino acid composition and type of fatty acid chains. Here, we report a new pelgipeptin peptide (Pelgipeptin L, PGP-L) produced by Paenibacillus elgii BC34-6. The primary sequence of the purified PGP-L was determined by MS/MS analysis, and the secondary structural property was investigated using CD spectroscopy. The complete molecular structure of PGP-L was determined by 2D NMR spectroscopy. The sequence of PGP-L consists of 1Dab-2Val-3Dab-4Phe-5Leu-6Dab-7Val-8Leu-9Ser and a saturated linear fatty acid chain (-CH3CH2CH2CH3). In addition, the antimicrobial activity of PGP-L was determined by minimal inhibitory concentration (MIC). The mechanism of action of PGP-L was investigated by fluorescence experiments. In summary, PGP-L was highly active against Gram-negative, Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) with 2-8 of MICs (µg/mL). The calcein dye leakage and membrane depolarization profiles of PGP-L exhibited a gradual increase in the fluorescence intensity, which suggests that PGP-L is targeting bacterial membrane by membrane disruption. PGP-L showed lower hemolysis activity than other pelgipeptin peptides. Taken together, we suggest that PGP-L can be a potential antibiotics with low cytotoxicity.

  1. X. C. Wu, X. B. Shen, R. Ding, C. D. Qian, H. H. Fand, and O. Li, “ Isolation and partial characterization of antibiotics produced by Paenibacillus elgii B69”, FEMS Microbiol Lett. 2010, 310, 32.
  2. R. Ding, W. C. Wu, C. D. Qian, Y. Teng, O. Li, Z. J. Zhan, and Y. H. Zhao, “Isolation and Identification of Lipopeptide Antibiotics from Paenibacillus elgii B69 With Inhibitory Activity Against Methicillin-Resistant Staphylococcus aureus”, J. of Microbiolo. 2011, 49, 942.